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cdna synthesis kit superscript choice system for cdna synthesis  (Thermo Fisher)


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    Thermo Fisher cdna synthesis kit superscript choice system for cdna synthesis
    Cdna Synthesis Kit Superscript Choice System For Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+choice+system+cdna+synthesis+kit/high+capacity+cdna+reverse+transcription+kit/pmc05840397-127-6-15
    Average 90 stars, based on 1 article reviews
    cdna synthesis kit superscript choice system for cdna synthesis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: The Fruitless gene in Nasonia displays complex sex-specific splicing and contains new zinc finger domains.
    Article Snippet: .. Construction and Screening of Sex-Specific cDNA Libraries cDNA libraries were prepared with the SuperScript Choice System for cDNA Synthesis kit (Invitrogen) followingmanufacturer’s instructions. .. PolyAþRNApurified from heads of 1- to 2-day-old adult N. vitripennis males and females was reverse transcribed with oligodT and random hexamer primers in separate reactions (2.5 lg polyAþ RNA for each reaction) and pooled after second strand synthesis.Double-stranded cDNAswere cloned into pSMARTcDNA plasmids and electroporated into Lucigen’s E. cloni cells following the manufacturer’s instructions (Lucigen, Middleton, WI). cDNA libraries were screened according to standard techniques (Ausubel et al. 2003).

    Article Title: Target Discovery and Validation in Pancreatic Cancer
    Article Snippet: C7] 3′ NEB T4 Polynucleotide Kinase (10U/μl; NEB #201S) The 12% PAGE-TAE gel may be made from the following: 40% Polyacrylamide (19:1 acrylamide:bis) (Bio-Rad #161-0144) 50X Tris-Acetate-EDTA (TAE) buffer Quality Biological #330-008-161) (See note 1 ) Step 2 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Dynabeads oligo (dT)25 mRNA direct kit (Dynal, #610.11). .. Dynal Magnetic Particle Concentrators (Dynal MPC-S #120.20) Superscript Choice System cDNA Synthesis Kit (Gibco BRL #18090-019) 0.5M EDTA Sigma # E7889 Sodium dodecyl sulfate (SDS) (Sigma L4390) Step 3 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Nla III (NEB, #125S) Step 4 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 T4 ligase (high concentration = 5U/μl; Life Technologies #15224-041) BSA (Bovine Serum Albumin) (NEB #B9001S) Step 5 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 BmsfI (NEB#572S) Phenol-Chloroform 0.5M EDTA. .. Sigma #P2069 LoTE = 3mM Tris-HCl (pH 7.5) + 0.2mM EDTA (pH 7.5) Step 6 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Klenow 2U/μl (Pharmacia #27-0929-01) 7.5M ammonium acetate (Sigma #A2706) Step 7 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 T4 Ligase (High Concentration 5U/μl; Life Technologies #15224-041) Step 8 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Primer 1 5′ GGA TTT GCT GGT GCA GTA CA 3′ Primer 2 5′ CTG CTC GAA TTC AAG CTT CT 3′ 40% Polyacrylamide (19:1 acrylamide:bis) (Bio-Rad Cat No. 161-0144) for making 12% PAGE-TAE gel 96 well plates for PCR (Sigma #Z37,490-3) Taq polymerase (BRL #10996-034) Step 9 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 SYBR Green I (Molecular Probes S-7567) SpinX filtration microcentrifuge tubes (Costar Corp, Cambridge, MA) Step 10 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 TE (10mM Tris-HCl (pH 7.5), 1mM EDTA (pH 8.0) Step 11 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 40% polyacrylamide (37.5:1 acrylamide:bis) (Bio-Rad #161-0148) Step 12 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 SphI restriction enzyme (NEB #182S) PZero plasmid (Invitrogen #K2500-01) Step 13 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 ElectroMAX DH10B competent cells Zeocin (Invitrogen # R250-01) Glass beads Agarose (Gibco BRL Select Agar #30391-023) 3.2.

    Article Title: Id1 is a common downstream target of oncogenic tyrosine kinases in leukemic cells
    Article Snippet: .. Total RNA (10 μg) was reverse-transcribed with Superscript Choice System cDNA synthesis kit (Invitrogen) in the presence of an oligodT-T7 primer (GENSET, La Jolla, CA). .. The cDNA then used for in vitro transcription with the BioArray Highyield RNA Transcript Labeling Kit (ENZO Diagnostics, Farmingdale, NY).

    Article Title: New insights into clinical trial for Colostrinin in Alzheimer's disease.
    Article Snippet: Background: The pathomechanism of Alzheimer’s disease (AD) is multifactorial although the most popular hypotheses are centered on the effects of the misfolded, aggregated protein, amyloid beta (A ) and on Tau hyperphosphorylation.. Objectives: Double blinded clinical trials were planned to demonstrate the effect of ColostrininTM (CLN) on instrumental daily activities of AD patients.. The potential molecular mechanisms by which CLN mediates its effects were investigated by gene expression profiling.

    Article Title: Effects of Colostrinin on gene expression-transcriptomal network analysis.
    Article Snippet: Article history: ColostrininTM (CLN) is a unif Received 15 May 2008 Received in revised form 23 October 2008 Accepted 27 October 2008

    Article Title: Comparative gene expression of intestinal metabolizing enzymes.
    Article Snippet: .. TRIzol reagent and superscript Choice System for cDNA synthesis kit were purchased from Invitrogen (Carlsbad, CA). .. The BioArray high-yield RNA transcript labeling kit was purchased from Enzo Biochem (New York, NY).

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    Concentration Assay:

    Article Title: Target Discovery and Validation in Pancreatic Cancer
    Article Snippet: C7] 3′ NEB T4 Polynucleotide Kinase (10U/μl; NEB #201S) The 12% PAGE-TAE gel may be made from the following: 40% Polyacrylamide (19:1 acrylamide:bis) (Bio-Rad #161-0144) 50X Tris-Acetate-EDTA (TAE) buffer Quality Biological #330-008-161) (See note 1 ) Step 2 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Dynabeads oligo (dT)25 mRNA direct kit (Dynal, #610.11). .. Dynal Magnetic Particle Concentrators (Dynal MPC-S #120.20) Superscript Choice System cDNA Synthesis Kit (Gibco BRL #18090-019) 0.5M EDTA Sigma # E7889 Sodium dodecyl sulfate (SDS) (Sigma L4390) Step 3 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Nla III (NEB, #125S) Step 4 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 T4 ligase (high concentration = 5U/μl; Life Technologies #15224-041) BSA (Bovine Serum Albumin) (NEB #B9001S) Step 5 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 BmsfI (NEB#572S) Phenol-Chloroform 0.5M EDTA. .. Sigma #P2069 LoTE = 3mM Tris-HCl (pH 7.5) + 0.2mM EDTA (pH 7.5) Step 6 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Klenow 2U/μl (Pharmacia #27-0929-01) 7.5M ammonium acetate (Sigma #A2706) Step 7 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 T4 Ligase (High Concentration 5U/μl; Life Technologies #15224-041) Step 8 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Primer 1 5′ GGA TTT GCT GGT GCA GTA CA 3′ Primer 2 5′ CTG CTC GAA TTC AAG CTT CT 3′ 40% Polyacrylamide (19:1 acrylamide:bis) (Bio-Rad Cat No. 161-0144) for making 12% PAGE-TAE gel 96 well plates for PCR (Sigma #Z37,490-3) Taq polymerase (BRL #10996-034) Step 9 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 SYBR Green I (Molecular Probes S-7567) SpinX filtration microcentrifuge tubes (Costar Corp, Cambridge, MA) Step 10 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 TE (10mM Tris-HCl (pH 7.5), 1mM EDTA (pH 8.0) Step 11 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 40% polyacrylamide (37.5:1 acrylamide:bis) (Bio-Rad #161-0148) Step 12 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 SphI restriction enzyme (NEB #182S) PZero plasmid (Invitrogen #K2500-01) Step 13 list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 ElectroMAX DH10B competent cells Zeocin (Invitrogen # R250-01) Glass beads Agarose (Gibco BRL Select Agar #30391-023) 3.2.

    Reverse Transcription:

    Article Title: Id1 is a common downstream target of oncogenic tyrosine kinases in leukemic cells
    Article Snippet: .. Total RNA (10 μg) was reverse-transcribed with Superscript Choice System cDNA synthesis kit (Invitrogen) in the presence of an oligodT-T7 primer (GENSET, La Jolla, CA). .. The cDNA then used for in vitro transcription with the BioArray Highyield RNA Transcript Labeling Kit (ENZO Diagnostics, Farmingdale, NY).

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    In Vitro:

    Article Title: Effects of Colostrinin on gene expression-transcriptomal network analysis.
    Article Snippet: Article history: ColostrininTM (CLN) is a unif Received 15 May 2008 Received in revised form 23 October 2008 Accepted 27 October 2008

    Labeling:

    Article Title: Effects of Colostrinin on gene expression-transcriptomal network analysis.
    Article Snippet: Article history: ColostrininTM (CLN) is a unif Received 15 May 2008 Received in revised form 23 October 2008 Accepted 27 October 2008

    other:

    Article Title: Method of modulating inflammatory response
    Article Snippet: First-strand cDNA was transcribed from total RNA using T7-(dT)24 oligomer primer and SSII reverse transcriptase at 37° C. The second strand cDNA is synthesized from first-strand cDNA using DNA ligase, DNA polymerase I and T4 DNA polymerase at 16° C. (SuperScript Choice System for cDNA synthesis kit, Gibco), and then cleaned with Phase-Locking gel.

    Polymerase Chain Reaction:

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    Acrylamide Gel Assay:

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    SYBR Green Assay:

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    TA Cloning:

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    Sequencing:

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    Plasmid Preparation:

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.

    Agarose Gel Electrophoresis:

    Article Title: Serial analysis of gene expression (SAGE): experimental method and data analysis.
    Article Snippet: This unit provides a protocol for performing serial analysis of gene expression (SAGE).. SAGE involves the generation of short fragments of DNA, or tags, from a defined point in the sequence of all cDNAs in the sample analyzed.. This short tag, because of its presence in a defined point in the sequence, is typically sufficient to uniquely identify every transcript in the sample.



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